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Bacteriology Section: General Bacteriology
Laboratory |
 Automated bacterial identification and antimicrobial susceptibility equipment used in the routine bacteriology laboratory. |
| and eggs from poultry houses in the state. This important food safety program has been instrumental in decreasing the number of cases of food-borne salmonellosis in people in the Northeastern United States. The PEQAP program is currently being used as a template for a national egg safety program that is being developed. The SE section also participates in research projects that help to validate the PEQAP program, and help to determine better methods of detection of S. enteritidis in poultry and poultry environments. The mastitis section performs culture on bulk tank milk and quarter samples from cows with mastitis. Herd surveys are | |
| often done to determine the extent of infection with mastitis pathogens within a herd. This section works closely with the extension service group in the Department of Veterinary Science. The molecular diagnostics section analyzes isolates of E. coli and Clostridium perfringens for the presence of virulence factors and toxins by using Polymerase Chain Reaction (PCR) techniques. This section also uses PCR to detect Mycoplasma species in clinical samples, such as milk and porcine lungs. This section is also involved in several research projects that involve identifying putative pathogens in disease syndromes, or developing better diagnostic tests for organisms such as S. enteritidis. |
 Staphylococcus chromogenes growing on a blood agar medium. |
Bacteriology Section Personnel: |
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Brenda C. Love: DVM, PhD, diplomate ACVM: section head. Dr. Love received her DVM from Oklahoma State University, and her PhD in veterinary microbiology from the University of California at Davis. Areas of interest include antimicrobial resistance development by bacteria, especially enteric bacteria (Salmonella, E. coli); salmonellosis and Johne's disease in dairy cattle; SE infection of poultry and contamination of eggs; and molecular diagnostic testing. Valerie Lintner: routine bacteriology senior technician. Performs routine culture (aerobic, anaerobic, fungal, mycoplasma) and susceptibility testing. Amanda Layman: mastitis technician. Performs bulk tank quantitative culture, routine quarter culture, fungal, and mycoplasma culture, and antimicrobial susceptibilities. Traci Pierre: SE senior technician. Performs culture for PEQAP program. Coordinates activities with PEQAP administrator.
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 Identification of Salmonella enteritidis positive birds by ELISA |
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Waslohn: Bacteriology and Parasitology Technician. Assists
in SE, mastitis, and routine bacteriology areas and performs
parasitology examinations.
Suzanne Myers: Molecular diagnostics technician, research technician. Performs PCR assays for toxin typing of Clostridium perfringens and detection of Mycoplasma sp. Performs molecular studies for research grants. Frank Zambito: Bacteriology Technician. Works in routine bacteriology and mastitis areas; assists with research projects. |
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Specific Procedures Performed by Section: |
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Aerobic culture Anaerobic culture Antibiotic residue, LAST (urine) Antibiotic susceptibility testing Blackleg assay (FA) Brachyspira culture Campylobacter culture Clostridium perfringens culture Cl. perfringens PCR (toxin typing) E. coli serotyping/virulence testing Fungal culture Leptospira assay (FA) Mastitis culture
Mycoplasma PCR Quantitative bacterial culture Salmonella culture
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